grinder for cereals mycotoxins determination

  • List of CEN, EN ISO methods general requirements for

    mycotoxins CEN/TR 15298:2006: Foodstuffs - Sample comminution for mycotoxins analysis Comparison betwe- en dry milling and slurry mixing Food . General requirements for all mycotoxins CEN/TR 16059:2010: Food analysis - Performance criteria for single laboratory validated methods of analysis for the determination of mycotoxins . Cereals, shell

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  • Determination of the Mycotoxins Zearalenone,

    mycotoxin, the mycotoxin binds with colored beads in the lateral flow test strip and the color intensity in the test and control zones is measured by the Mycomaster reader and displayed as ppb (parts per billion) which is the same as g/kg. 3.Materials 3.1 Equipment 3.1.1 Mycomaster reader 3.1.2 Mini-centrifuge 3.1.3 Grinder (Retsch or coffee

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  • Simple methodology for the determination of mycotoxins

    Simple methodology for the determination of mycotoxins in pseudocereals, spelt and rice Natalia Arroyo-Manzanares1, Jos F. Huertas-Prez1, Ana M. Garca-Campaa, Laura Gmiz-Gracia* Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva s/n, E-18071 Granada, Spain

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  • AB SCIEX The Quantitation of Mycotoxins in Cereals Using a

    p 1 The Quantitation of Mycotoxins in Cereals Using a Simple Sample Extraction and LC-MS/MS with Fast Polarity Switching and the Scheduled MRM™ Algorithm Jianru Stahl-Zeng1, Stephen Lock2, 1Stefanie Kreppenhofer1, and Kristin von Czapiewski 1 AB SCIEXD arms tad (Germany); 2

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  • Simultaneous determination of 16 mycotoxins in cereals

    A reliable quantitative method for the rapid and simultaneous determination of 16 mycotoxins commonly found in major cereal species has been established using an Agilent Triple Quadrupole LC/MS system. This method can meet the requirements for high-throughput and rapid monitoring of multiple mycotoxins commonly found in cereals. Experimental

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  • Preparation of Samples for Aflatoxin Testing

    Preparation of Samples for Aflatoxin Testing. Aflatoxins are produced by various types of moulds which can grow on foodstuffs such as pistachio nuts, peanuts, cereals, dried fruits, etc. Much of the information in this report can also be applied to testing for other mycotoxins and ochratoxins.

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  • Determination of multi

    Determination of multi-mycotoxin occurrence in cereals, nuts and their products in Cameroon by liquid chromatography tandem mass spectrometry (LC-MS/MS)

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  • Determination of 17 Mycotoxins in Cereals and Cereal

    Determination of Mycotoxins in Cereals and Cereal ased ood Usin Liquid Chromatoraphy–Triple Quadrupole Mass Spectrometry atharina von argen, Ebru Sariaya, and Michal odula Thermo Fisher Scientic, Special Solutions Center Europe, reieich, ermany Application Note 646 Key Words Commission Regulation 1881/2006/EC, mycotoxin detection

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  • Monitoring Food Safety in Armenia. Assessment

    Apr 05, 2014The data of distribution for mycotoxins in wheat/barley grains by provinces (table 2) revealed the large scatter values of the occurrence of OTA and T-2 toxin, including contaminated and mycotoxin-free samples of cereals, which partially, could be considered as result of the impact of climatic features.

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  • Determination of Ochratoxin A in Selected Cereal Grains

    Determination of Ochratoxin A in Selected Cereal Grains Retailed in Nairobi County, Kenya belongs to a group of mycotoxins which are a key threat to quality of cereals based foodstuff. Mycotoxins are toxic, carcinogenic, nephrotoxic, neurotoxic and immunotoxic secondary metabolites cereals, HPLC, Kenya, mycotoxins, Ochratoxin A. 1

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  • INCIDENCE OF FUSARIUM SPECIES AND ITS MYCOTOXINS IN

    cereals (maize, wheat, barley and oat) collected from different areas of Banat. Fungal contamination degree was realised using the direct determination method for the total number of fungi colonies, and the mycotoxins concentration was determined with the immune-enzymatic method ELISA. The obtained results are similar with the

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  • Mycotoxins in the Grain Market

    Jan 08, 1993Mycotoxins are poisonous trace organic residues of mold deterioration. Mycotoxins are not alive themselves, but instead are very potent compounds causing, at very low dosages (parts per million (ppm) or parts per billion (ppb)), a variety of human and animal health problems. Individual mycotoxins are produced by specific mold strains under more restrictive growth conditions

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  • Determination of mycotoxins in Food by LC and LC/MS

    Mycotoxins are secondary metabolites produced by certain (cereals, nuts, milk, and coffee). The classes of mycotoxins that frequently are found in foods are aflatoxins, ochratoxins, fumonisins, trichothecenes Determination of mycotoxins in Food by LC and LC/MS

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  • Review Article Mycotoxin Analysis: New Proposals for

    for the multiclass determination of mycotoxins in a great variety of food commodities (some of them scarcely studied), such as cereals, pseudocereals, cereal syrups, nuts, edible seeds, and botanicals. Considering the latest technical developments, ultrahigh

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  • Human exposure to mycotoxins and their masked forms

    Highlights An assessment of exposure of the population to (masked) mycotoxins was performed. A deterministic and probabilistic exposure assessment was executed. Mean mycotoxin intakes for all cereal-based foods were beneath tolerable daily intake. Sub-populations can be exposed to high levels of zearalenone-equivalents. Systematic monitoring of all mycotoxins is a prerequisite to reach safety

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  • Rapid surface plasmon resonance immunoassays for the

    In recent times surface plasmon resonance has demonstrated its applicability to the detection of a wide range of contaminants in food and feed including mycotoxins in cereals and cereal-based food products. Commercially available, laboratory-based systems have exploited high affinity polyclonal, monoclonal and recombinant antibodies and robust sensing surfaces to provide rapid, accurate and

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  • Advanced LC–MS

    rence and determination of mycotoxins have been published [11, 13, 16, 20–24]. Moreover, new developments and up-dates in this field are covered on a yearly basis inWorld Mycotoxin Journal [25–28]. This review thus provides insight into LC–MS-based methods for the determination of co-occurring mycotoxins.

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  • Simultaneous Determination of Twelve Mycotoxins in Cereals

    A collaborative study to validate novel field immunoassay kits for rapid mycotoxin detection. Int J Food Microbiol, 2002, 75: 135–142 [5]Tanaka T, Yoneda A, Inoue S, Sugiura Y, Ueno Y. Simultaneous determination of trichothecene mycotoxins and zearalenone in cereals by gas chromatography–mass spectrometry. J Chromatogr A, 2000, 882: 23–28

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  • Chromatographic methods for the simultaneous determination

    Masked mycotoxins: determination of a deoxynivalenol Methods for the simultaneous determination of several glucoside in artificially and naturally contaminated wheat by liquid groups of mycotoxins are highly desirable. comparison analysis of several Fusarium mycotoxins in cereals by capillary gas and convenient determination by gradient

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  • Analysis of cocoa products for ochratoxin A and aflatoxins

    curves as the coefficient of determination (R2) was above 0.999 for each mycotoxin. Fortified (spiked) samples of cocoa products were pre-pared by adding an aliquot of each spiking solution (50 ng/ml OTA in methanol and 20 ng/ml AFB 1, AFB 2, AFG 1, and AFG 2 in toluene) to 5-g ground sample, and extracting after 15 min.

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  • Thin Films Sensor Devices for Mycotoxins Detection in

    2. Conventional Analytical Methods for Mycotoxins Detection Chromatography is the most commonly used method used for mycotoxin analysis in food and feed [29]. The thin layer chromatography (TLC) was the first chromatographic method to be applied for mycotoxin determination and, nowadays, is still a routine technique used in several laboratories.

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  • DETECTION METHODS FOR MYCOTOXINS IN CEREAL

    DETECTION METHODS FOR MYCOTOXINS IN CEREAL GRAINS AND CEREAL PRODUCTS ABSTRACT: Analytical methods for mycotoxins in cereals and cereal-based products require three major steps, including extraction, clean-up (to eliminate interferences from the extract and concentrate the analyte), and detection/determination of the toxin (by using

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  • Fusarium Mycotoxin Content Of Slovakian Organic And

    production, results show significantly lower contamination of DON mycotoxins than in conventional production. Average DON content within observed period in wheat from RUJDQLFSURGXFWLRQUDQJHGI URP J NJ-1 in 2009 to J NJ-1 in 2010. 167 Remža et al.: Fusarium Mycotoxin Content Of Slovakian Organic And Conventional Cereals

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  • Chapter 23 : Mycotoxins

    Abstract: This chapter talks about specific mycotoxins that are produced only by specific fungi, usually by only a few species. A particular species of fungus may produce more tha

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  • Single laboratory

    determination in cereal products. These methods involve preliminary solvent extraction and clean-up on specific immunoaffinity columns. The pro-cedures were validated with respect to general requirements of single laboratory validation. In addition, the methods were applied to determine the mycotoxins contamination in cereal foods

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  • TOOLS FOR FUSARIUM MYCOTOXIN REDUCTION IN FOOD

    TOOLS FOR FUSARIUM MYCOTOXIN REDUCTION IN FOOD AND FEED CHAINS coefficient of determination (R2) between these, Grinder) without sieving as described by Yli-Mattila et al. (2013). DNA was extracted from ground grain samples and from the F. graminearum isolate G243

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  • Determination of Aflatoxins in Wheat and Wheat by

    Determination of Aflatoxins in Wheat and Wheat by-products Intended for Human Consumption, Marketed in Rio de Janeiro, Brazil. Felipe Machado Trombete 1,, Douglas de vila Moraes 2, Yuri Duarte Porto 1, Thas Barbosa Santos 1, Glria Maria Direito 3, Marcelo Elias Fraga 3, Tatiana Saldanha 2. 1 Department of Food Technology, Institute of Technology, Federal Rural University of Rio de

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  • Simultaneous determination of multi

    12. Institute for Reference Materials and Measurements, Food Safety and Quality - Geel, Belgium: Method for the simultaneous determination of deoxynivalenol, HT-2 toxin, T-2 toxin and zearalenone in unprocessed cereals and cereal-based compound animal feeds, 2011.

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  • 1. Introduction

    Mycotoxins may also be found in cereal syrups as result of using contaminated raw material, or because of contamination of the final manufactured product by microorganisms during storage [53]. However, there are very few methods for the determination of mycotoxins in these matrices.

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  • Simultaneous determination of major type A and B

    A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the Fusarium mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3-glucoside, HT-2-3-glucoside, nivalenol-3

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